PepSep®

Smile! Liquid chromatography for MS made simple

High-performance, ready-to-use columns and emitters for maximum sensitivity and throughput

The challenge:

Achieving both depth and speed in LC-MS proteomics is often a balancing act. Conventional columns can lead to high backpressure, inconsistent performance, and time-consuming setups — especially when handling large sample cohorts or complex matrices.

The solution:

PepSep® columns and emitters are designed to deliver outstanding sensitivity, reproducibility, and robustness while remaining easy to use. Whether you are building deep spectral libraries or focusing on high-throughput studies, the PepSep® portfolio offers plug-and-play solutions to streamline your workflows.

Apply! Simple, streamlined workflows

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Results

Identified unique peptide and protein groups, from 0.25, 1, 4 and 16 ng HeLa Tryptic Digest injection on an PepSep® Advanced column (25 cm x 75 µm x 1.5 µm) and Competitor A Column (pulled tip column, 25 cm x 75 µm x 1.7 µm). Samples were run on a nanoElute® 2 coupled to a Bruker timsTOF Ultra 2, dia-PASEF® acquisition. Data analysed using DIA-NN 1.8.1.

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PreOmics®'

PepSep®

values

Smile

Outstanding data quality

Exceptional sensitivity for low-abundance peptides
Sharp, consistent peak shapes and stable chromatography
Flexible column formats for any workflow — from long gradients to ultra-fast runs

Relax

Simple, time-saving setup

Plug-and-play columns with easy connection
Separate, exchangeable emitters to minimize downtime
Robust performance across thousands of injections

Realize

Reliable, high-throughput results

Lower backpressure for faster operation
Consistent reproducibility for large sample sets
Optimized for cutting-edge LC-MS applications

PepSep® Columns & Emitters

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PepSep®

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Instrument

High-performance, ready-to-use columns and emitters for maximum sensitivity and throughput

PepSep®

Request quote

Instrument

High-performance, ready-to-use columns and emitters for maximum sensitivity and throughput

PepSep®

Request quote

Instrument

High-performance, ready-to-use columns and emitters for maximum sensitivity and throughput

PepSep® Classics* – Performance Meets Simplicity

Versatile columns for standard and advanced proteomics workflows.

50 cm × 75 µm × 1.5 µm → Spectral Library Generation‍
25 cm × 75 µm × 1.5 µm → Sensitivity
25 cm × 150 µm × 1.5 µm → Versatility
15 cm × 150 µm × 1.5 µm → Throughput
8 cm × 150 µm × 1.5 µm → Speed

* additional column dimensions available. The full list of dimensions can be found in the brochure

PepSep® Advanced – Next Level Sensitivity

For the highest performance and maximum peptide coverage.

25 cm × 75 µm × 1.5 µm → New gold standard

CaptiveSpray 2 Emitters

Plug-and-play emitters for hassle-free CaptiveSpray setup. Two variants, separate from columns for easy replacement and longer LC-MS system life with peak performance.