Smile! High throughput sample preparation simplified

Fast, reliable, standardized methods for bottom-up proteomic sample preparation on positive pressure devices

The challenge:

As LC-MS instrumentation has developed, there is a growing need to increase sample throughput and sample preparation can become the rate-limiting step.

The solution:

The PreOmics iST-PSI workflow couples the simplicity of the iST technology with positive pressure plate processing. Positive pressure processing is known to improve recovery and reproducibility compared to negative pressure or centrifugation. The format is easily automated on major liquid handling platforms including Tecan Resolvex A200 and Hamilton [MP]2.

Apply! Simple, streamline workflows

1. Sample
Samples should be liquid, homogenates, or soft pellets.
2. Lyse, reduce, alkylate
Add LYSE reagent, and incubate for 10 mins at 95°C.
3. Digest
Cool, add DIGEST reagent, and incubate for 60 mins at 37°C.
4. Processing on positive pressure device
Load onto SPE plate. WASH and ELUTE on a positive pressure device. Dry and resuspend in LOAD.
5. MS analysis
Inject and analyze to see improved peptide identities.

Results

Time flies with PreOmics iST-PSI sample preparation

While standard proteomics sample preparation takes up to 44 hours – the same time as flying around the world non-stop, PreOmics kits take the same time as flying from our Munich HQ to London or from Boston to Chicago.  Increase your sample throughput, improve quality and produce reliable data. It’s as easy as i-S-T!

PreOmics'

iST-PSI

values

Smile

Improved sample quality
  • High reproducibility: Pearson correlation > 0.93
  • Working range: 1-100 µg
  • Increased peptide and protein  identifications
  • Shipped at RT🌿

Relax

High throughput sample preparation
  • High throughput sample preparation: 96 samples within 3 hours
  • Applications notes available for popular liquid handling platforms
  • Can be used on manual positive pressure manifolds

Realize

Cleaner peptides ready for analysis
  • Dual clean up step removes hydrophilic and hydrophobic contaminants
  • Achieve ready-to-measure peptides
  • Reduce mass spectrometry downtime
  • Gain greater benefit from your installed automation platform

iST-PSI

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Instrument
Instrument
Fast, reliable, standardized methods for bottom-up proteomic sample preparation on positive pressure devices
Instrument
Instrument
Fast, reliable, standardized methods for bottom-up proteomic sample preparation on positive pressure devices
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iST-REG-PSI 96HT (192rxn)

P.O.00108
192 sample kit
 $5,074 
4.227 €
Complete iST sample preparation with 2 clean-up runs with 96 samples each on positive pressure devices

iST-REG-PSI Buffer Add-on

P.O.00109
Buffer Add-on
 $545 
449 €
Buffers in amber bottles for additional clean-up runs
The products displayed are for visual representation only and may differ from those received

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Applying the science
Find out more about the wide range of applications and sample types to which PreOmics technology has been successfully applied