Smile! Protein and peptide sample preparation automated

Application for high throughput proteomic sample preparation for LC-MS analysis

The challenge:

How do you improve reproducibility and hands on time savings in proteomics sample preparation for LC-MS analysis when you have already addressed the challenges of time, sample contamination and loss?

The solution:

Automate! Together, PreOmics® and Hamilton®* have developed a completely automated high throughput LC-MS sample preparation workflow, combining the Hamilton® liquid handling technology with the PreOmics® iST-PSI workflow. To meet the demands of high-throughput proteomics sample preparation, this fully automated, efficient and reproducible workflow helps decrease the processing time to about 4 hours and produces high quality samples for LC-MS analysis!

*“Hamilton” is a registered Trademark of a third party

Apply! Simple, streamlined workflows

1. Adding samples
Samples should be liquid, homogenates or a soft pellet.
2. iST-PSI kit setup
Set up the iST-PSI consumables on the HamiltonMicrolab® VANTAGE Liquid Handling System®.
3. Sample preparation
Run pre-defined protocols in consultation with your automation specialist.
4. MS analysis
Analyze samples by LC-MS.
5. Data analysis
Process data to see improved peptide identities.

Results

Figure 1: Inter-day reproducibility and comparison to manual processing.(A and B) 8 aliquots of manually prepared P. pastoris protein extracts(50 µg each) were processed on two different days, and 4 additional samples were digested with the manual procedure.(C and D) 8 samples of commercially available human plasma (each~70 µg; Sigma-Aldrich, P9523) were processed on day 1 and 2.
Figure 2: 96 sample run –  48 aliquots of manually extracted P. pastoris proteins were processed together with 48 samples of commercially available human plasma.

More detailed explanation about these results can be found on the Hamilton® application note.

PreOmics®'

Hamilton

values

Smile

Improved sample quality
  • High reproducibility: Pearson correlation > 0.93
  • Working range: 1-100 µg
  • Increased peptide and protein  identifications

Relax

Fully automated high throughput sample preparation
  • Set up and walk away, hands-free LC-MS sample preparation
  • Fully flexible, process 1 to 96 sample
  • Minimal tip usage

Realize

Cleaner peptides ready for analysis
  • Dual clean up step removes hydrophilic and hydrophobic contaminants
  • Achieve ready-to-measure peptides
  • Reduce mass spectrometry downtime
  • Standardized workflow with high data reproducibility and process safety

Hamilton

Request quote
Cart
0
Instrument
Instrument
Application for high throughput proteomic sample preparation for LC-MS analysis
Instrument
Instrument
Application for high throughput proteomic sample preparation for LC-MS analysis
Request quote
No items found.
The PreOmics® products displayed are for visual representation only and may differ from those received
Imagine
Applying the science
Find out more about the wide range of applications to which PreOmics technology has been successfully applied