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The challenge:
How do you automate sample preparation of very dirty samples or where harsh denaturation reagents are needed? Typically these samples cause issues with proteomic sample preparation as the reagents can adversely impact digestion and subsequent LC-MS analysis.
The solution:
Use the combined advantages of SP3® and iST! Together, PreOmics® and Tecan®* have developed an automated LC-MS sample preparation workflow, combining the ability to wash and digest samples when bound to the SP3® beads and then do the clean-up step. This application meets the demands of efficient, robust proteomics sample preparation for difficult samples for LC-MS analysis with the bonus that an automated workflow helps labs decrease the hands-on time and produce high quality samples for LC-MS analysis!
*“Tecan” is a registered Trademark of a third party
Apply! Simple, streamlined workflows
Results
Additional data can be found in the Tecan® SP3-iST application note. The numbers of PSMs and IDs for both peptides and proteins were very consistent for all samples, regardless of protein input, as well as between the manual and the automated protocols. Approximately 27,370 peptides and 2,960 proteins were identified in all samples, in agreement with the expected performance of the nanoLC-MS measurements conducted. This demonstrates the high efficiency, quality and robustness of the automated SP3-iST workflow for the entire range of tested input amounts (15-50 μg).
PreOmics®'
Tecan
values
Smile
- Working range: 1-100 µg
- Good consistency between manual and automated workflows
- Excellent missed cleavage rate
Relax
- Benefit from combined SP3® and iST technology
- Set up and walk away, hands-free LC-MS sample preparation
- Fully flexible, process 1 to 96 samples
Realize
- Standardized workflow with high data reproducibility
- Dual clean up step removes hydrophilic and hydrophobic contaminants
- Achieve ready-to-measure peptides
Tecan
Tecan
