Smile! Plasma and serum sample preparation simplified

Fast, reliable, and reproducible solution for the dynamic range challenge in plasma and serum samples with scalable throughput

The challenge:

Accessing low-abundant proteins of interest in plasma and serum samples is challenging. The high dynamic range in protein concentrations and the heterogeneity and complexity pose significant obstacles for LC-MS-based proteomics, limiting access to full proteome information. In consequence, results lack robustness and reproducibility, traditional workflows are complex and time-consuming and low-abundant proteins are masked by high-abundant proteins. Thus, the impracticality of automation, the lack of flexibility and the inability to produce unbiased results impede the application for large sample cohorts.

The solution:

The ENRICH-iST kit provides an easy-to-use, yet robust solution to the dynamic range challenge of plasma and serum samples. Low-abundant proteins are enriched onto paramagnetic beads while analytical depth and proteome coverage are conserved. With scalable throughput, exceptional reproducibility, and low CVs, the process takes only 5h from sample to pure peptides. ENRICH-iST is compatible with human samples and other mammalian species like mouse, rat, pig or dog. Thanks to the simple protocol and compatibility with established lab automation systems the processing of large sample cohorts becomes an easy to achieve reality.

Apply! Simple, streamlined workflows

1. Sample
Samples should be mammalian plasma or serum.
2. ENRICH
Add sample to prepared EN-BEADS and incubate for 30 min at 30°C.
3. Lyse, reduce, alkylate
Add LYSE reagent and incubate for 10 min at 95°C.
4. Digest
Cool, add DIGEST reagent, and incubate for 1 h at 37°C.
5. Purify
Load onto the cartridge, WASH, and ELUTE. Dry and resuspend in LOAD.
6. MS analysis
Inject and analyze to see improved peptide identities.

Results

Time flies with PreOmics® ENRICH-iST sample preparation

While standard proteomics sample preparation takes up to 44 hours – the same time as flying around the world non-stop, PreOmics® kits take the same time as flying from our Munich HQ to London or from Boston to Chicago. Increase your sample throughput, improve quality and produce reliable data. It’s as easy as i-S-T!

PreOmics®'

ENRICH-iST

values

Smile

Improved plasma and serum sample quality
  • Enrichment of low-abundant proteins to reduce dynamic range
  • High reproducibility: CV ~16 % for inter-day and inter-batch experiments
  • Low sample input: 20 µL plasma
  • Shipped at RT🌿

Relax

Fast and easy plasma sample preparation
  • Fast processing of plasma or serum to pure peptides within 5 hours
  • All-in-one solution with all needed reagents included
  • Easy preparation of 1 - 96 samples with standard lab equipment
  • Compatible with established lab automation systems

Realize

Solving the dynamic range challenge in plasma and serum samples
  • Highly efficient bead-based enrichment of low abundance proteins
  • Increased analytical depth and proteome coverage
  • Minimized artificial modifications and optimized alkylation rates
  • Flexible use of plasma and serum samples of humans and other mammalian species like mouse, rat, pig and dog

ENRICH-iST

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ENRICH-iST

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Instrument
Instrument
Fast, reliable, and reproducible solution for the dynamic range challenge in plasma and serum samples with scalable throughput

ENRICH-iST

Request quote
Instrument
Instrument
Fast, reliable, and reproducible solution for the dynamic range challenge in plasma and serum samples with scalable throughput
Request quote

ENRICH-iST 8x

P.O.00163
8-sample kit
 $659 
659 €

ENRICH-iST 96x

P.O.00164
96-sample kit
 $7,910 
7.910 €

ENRICH-iST 96x HT

P.O.00165
96-sample kit
 $7,910 
7.910 €
DIGEST buffer in 1 vial for high-throughput applications
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