Smile! Plasma, serum & CSF sample preparation simplified

Accessing low-abundant proteins of interest in plasma, serum, CSF and similar samples is challenging. The high dynamic range in protein concentrations and the heterogeneity and complexity pose significant obstacles for LC-MS-based proteomics, limiting access to full proteome information. In consequence, results lack robustness and reproducibility, traditional workflows are complex and time-consuming and low-abundant proteins are masked by high-abundant proteins. Thus, the impracticality of automation, the lack of flexibility and the inability to produce unbiased results impede the application for large sample cohorts.

The ENRICH-iST kit provides a robust, all-in-one solution to the dynamic range challenge in plasma, serum, CSF, and similar high-dynamic range samples. By enriching low-abundance proteins onto paramagnetic beads, it conserves analytical depth and proteome coverage while offering exceptional reproducibility (CV ~16%), scalability, and speed. With a simple protocol, the process transforms as little as 20 µL of plasma or serum into pure peptides in under 5 hours. Compatible with human and mammalian samples (e.g., mouse, rat, pig, dog) and adaptable to both manual and automated workflows, ENRICH-iST enables flexible, efficient, and reproducible processing of small-scale experiments and large sample cohorts alike.