Plan! Use PreOmics®' products for your research

Resources for working with biological fluids

Cell lines
Plant tissues
Biological fluids
Immunoprecipitations
Mammalian tissues
FFPE tissues
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Biological fluids

Biological fluids may be the starting point for a wide range of experimental investigations from discovery protein interaction studies, preclinical toxicology assessments, through to large clinical trial biomarker measurements. For example monitoring biomarkers in saliva is growing in interest because of the ease of collection from patients. Biofluids including plasma, serum, saliva, urine, CSF, synovia and others are compatible for processing with our iST or iST-BCT and iST-NHS kits.

The PreOmics® kits are suitable for use in research and regulated environments. The kits contain all you need for your peptide or protein sample preparation and are suitable for use by all members of your lab, no specialist experience is needed. The flexible formats of single samples or racked in to 96 well holders allow the kits to be used manually or with a range of automation options.

The catridges can be processed by centrifugation or using positive pressure manifolds, again giving options for all labs. Dedicated resources are shown below and our technical support team would be happy to help with advice.

Outline processing considerations: Samples with volumes of 1-11 µL can be directly mixed with appropriate LYSE buffer as described in the regular protocols. Volumes higher than 11 µL either require prior precipitation, concentration or mixing with our higher concentrated lysis buffers. Please ensure that the total protein present is within the range 1 – 100 µg.

Biological fluids may be the starting point for a wide range of experimental investigations from discovery protein interaction studies, preclinical toxicology assessments, through to large clinical trial biomarker measurements. For example monitoring biomarkers in saliva is growing in interest because of the ease of collection from patients. Biofluids including plasma, serum, saliva, urine, CSF, synovia and others are compatible for processing with our iST or iST-BCT and iST-NHS kits.

The PreOmics® kits are suitable for use in research and regulated environments. The kits contain all you need for your peptide or protein sample preparation and are suitable for use by all members of your lab, no specialist experience is needed. The flexible formats of single samples or racked in to 96 well holders allow the kits to be used manually or with a range of automation options.

The catridges can be processed by centrifugation or using positive pressure manifolds, again giving options for all labs. Dedicated resources are shown below and our technical support team would be happy to help with advice.

Outline processing considerations: Samples with volumes of 1-11 µL can be directly mixed with appropriate LYSE buffer as described in the regular protocols. Volumes higher than 11 µL either require prior precipitation, concentration or mixing with our higher concentrated lysis buffers. Please ensure that the total protein present is within the range 1 – 100 µg.

Resources

9:06
min
May 13, 2024
A step-by-step explanation on how to use the ENRICH-iST kit
The ENRICH-iST kit provides an easy-to-use, yet robust solution to the dynamic range challenge of plasma and serum samples.
Watch video
2:22
min
April 22, 2024
All you need to know about ENRICH-iST
One step forward in plasma (serum) sample preparation for MS-based proteomics with ENRICH-iST; an all-in-one solution based on the enrichment of low-abundance proteins onto paramagnetic beads and is compatible with human and other mammalian samples
Watch video
16:18
min
April 15, 2020
Step by step iST workflow for plasma and serum
Watch and learn how to optimize the processing of your plasma and serum samples with our iST workflow - a step-by-step explanation
Watch video
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Applying the science
Find out more about the wide range of applications to which PreOmics technology has been successfully applied