iST-S

Smile! Proteomics sample preparation simplified – even at low-input

Sensitive, high-throughput sample preparation for low-input samples

The challenge:

The rising demand for affordable, high‑throughput biopharma applications – such as large‑scale cell‑based screening and drug discovery – has accelerated adoption of low‑input proteomics. However, existing workflows struggle to deliver cost‑effective and scalable performance. While advances in MS sensitivity enable robust measurements from limited material, conventional sample preparation methods are not optimized for reliable high‑throughput processing at low inputs. As a result, insufficient peptide recovery remains a critical bottleneck, limiting proteome depth, quantitative accuracy, and data completeness.

The solution:

Built on proven in‑StageTip (iST) technology, the iST‑S sample preparation kit delivers a cost-effective solution for low‑input, high‑throughput proteomics. It maximizes peptide recovery and proteome depth, enabling meaningful biological insights from limited material. The ready‑to‑use kit supports fast, robust sample preparation in a 96‑well plate format within ~2 hours. Broad compatibility with LC‑MS systems and automation platforms ensures seamless integration into discovery, screening, and profiling workflows at scale.

Apply! Simple, streamlined workflows

Sample

Cells or pelleted protein samples with 1-10 µg protein input.

Lyse, reduce, alkylate

Premix LYSE A+B, add to the sample, incubate for 10 mins at 80°C.

Digest

Cool, add DIGEST reagent, and incubate for 60 mins at 37°C.

Purify

Load onto cartridge, WASH and ELUTE. Dry and resuspend in LOAD. Optional: cartridge pre-conditioning

5. MS analysis

Inject and analyze to see improved peptide IDs.

Results

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PreOmics^®'

iST-S

values

Smile

Improved performance & proteome depth at low inputs

Proven iST performance for low-input samples (1-10 µg)
Available as 96x plate-based workflow
Deep biological insights from limited material

Relax

Simple, clean and automated sample preparation

Robust & flexible workflow with minimal hands‑on time
Dual cleanup step removes hydrophilic and hydrophobic contaminants
Ready to measure peptides & reduced MS downtime
Compatible with third-party automation systems

Realize

Confident proteome profiling from low‑input cellular samples

Optimized for cellular proteomics, including cultured as well as primary cell material
Consistent proteome coverage across 1–10 µg protein input
Scalable for high‑throughput cell‑based studies and screening workflows
Reliable, high‑quality data enabling confident biological interpretation

PepSep® Columns & Emitters

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iST-S

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P.O.00259

Instrument

Sensitive, high-throughput sample preparation for low-input samples

iST-S

Request quote

P.O.00259

Instrument

Sensitive, high-throughput sample preparation for low-input samples

iST-S

Request quote

P.O.00259

Instrument

Sensitive, high-throughput sample preparation for low-input samples

PepSep® Classics* – Performance Meets Simplicity

Versatile columns for standard and advanced proteomics workflows.

50 cm × 75 µm × 1.5 µm → Spectral Library Generation | Art.-Nr. 1895846
25 cm × 75 µm × 1.5 µm → Sensitivity | Art.-Nr. 1893484
25 cm × 150 µm × 1.5 µm → Versatility | Art.-Nr. 1893476
15 cm × 150 µm × 1.5 µm → Throughput | Art.-Nr. 1893474
8 cm × 150 µm × 1.5 µm → Speed | Art.-Nr. 1893470

* additional column dimensions available. The full list of dimensions can be found in the brochure

PepSep® Advanced – Next Level Sensitivity

For the highest performance and maximum peptide coverage.

25 cm × 75 µm × 1.5 µm → New gold standard | Art.-Nr. 1919826

CaptiveSpray 2 Emitters

Plug-and-play emitters for hassle-free CaptiveSpray setup. Two variants, separate from columns for easy replacement and longer LC-MS system life with peak performance (Pack of 2 pieces).