Smile! Biological fluid sample preparation simplified

Fast, reliable, reproducible sample preparation minimizing artificial modifications

The challenge:

The multi-step methods of protein sample preparation for mass spectrometry analysis have inherent challenges of time, contamination and sample loss. When preparing biological fluids (e.g. plasma and serum) for proteomic analyses, harsh reagents may introduce artificial modifications resulting in reduced protein and peptide identifications.

The solution:

PreOmics’ iST-BCT sample preparation kit was developed to enable the robust processing of biological fluids and has been optimized to minimize artificial modifications (e.g. deamidation, oxidation) and increase the alkylation rate. Complex workflows previously requiring many different reagents over a two day time period are replaced by a single kit containing all standardized reagents to accomplish preparation for mass spectrometry protein analysis whether manually, semi or fully automated on the PreON in a 2 hour time window.

Apply! Simple, streamline workflows

1. Samples
Samples should be liquid, homogenates, or soft pellets.
2. Lyse, reduce, alkylate
Add LYSE reagent, and incubate for 10 mins at 95°C.
3. Digest
Cool, add DIGEST reagent, and incubate for 60 mins at 37°C.
4. Purify
Load onto the cartridge, WASH, and ELUTE. Dry and resuspend in LOAD.
5. MS analysis
Inject and analyze to see improved peptide identities.

Results

Time flies with PreOmics iST-BCT sample preparation

While standard proteomics sample preparation takes up to 44 hours – the same time as flying around the world non-stop, PreOmics kits take the same time as flying from our Munich HQ to London or from Boston to Chicago.  Increase your sample throughput, improve quality and produce reliable data. It’s as easy as i-S-T!

PreOmics'

iST-BCT

values

Smile

Improved sample quality
  • High reproducibility: R2> 0.9
  • Working range: 1-100 µg
  • Increased peptide and protein identifications
  • Shipped at RT🌿

Relax

Quicker methodology
  • 1 hour hands-on time
  • Single step incorporating lysis, enhanced reduction and alkylation
  • All-in-one solution, all reagents provided
  • Compatible with standard lab equipment

Realize

Cleaner peptides ready for analysis
  • Dual clean up step removes hydrophilic and hydrophobic contaminants
  • Achieve ready-to-measure peptides
  • Reduce mass spectrometry downtime
  • Manual to fully automated processing options available

iST-BCT

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Instrument
Instrument
Fast, reliable, reproducible sample preparation minimizing artificial modifications
Instrument
Instrument
Fast, reliable, reproducible sample preparation minimizing artificial modifications
Request quote

iST-BCT 8x

P.O.00084
8 sample kit
 $350 
325 €

iST-BCT 96x

P.O.00099
96 sample kit
 $3,070 
2.740 €
Racked into 96 well plate, has collars for individual processing

iST-BCT PreON 8x

P.O.00130
8 sample kit
 $407 
385 €
With PreON plasticware included

iST-BCT PreON 96x

P.O.00131
96 sample kit
 $3,550 
3.140 €
With PreON plasticware included
The products displayed are for visual representation only and may differ from those received

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Applying the science
Find out more about the wide range of applications and sample types to which PreOmics technology has been successfully applied