Chemical labeling reagents have specific handling and compatibility requirements to achieve good labeling efficiencies. Combined with the multi-step methods of proteomics sample preparation for mass spectrometry analysis, chemical labelling protocols have inherent challenges of time, contamination and sample loss. This challenge is exacerbated with large numbers of samples and small starting amount of input material.
PreOmics iST-NHS sample preparation is fully compatible with chemical labeling reagents. Allowing streamlined peptide processing, chemical labeling and an efficient peptide clean-up step. The iST-NHS method decreases hands-on time, sample loss and the amount of chemical label required. Complex workflows previously requiring many different reagents over a two day time period are replaced by a single kit containing all standardized reagents to accomplish proteomics sample labeling and preparation for mass spectrometry protein analysis whether manually, semi or fully automated on the PreON in a 2.5 hour time window.
While standard proteomics sample preparation takes around 44 hours – the same time as flying around the world non-stop, PreOmics kits take the same time as flying from our Munich HQ to London or from Boston to Chicago. Increase your sample throughput, improve quality and produce reliable data. It’s as easy as i-S-T!