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Meeting the challenges in mass spectrometry-based proteomics
Fast and efficient sample preparation technique for use in drug development and biomarker discovery, research and applied proteomics
The challenge
Common mass spectrometry (MS)-based proteomic workflows are multistep processes employing different methodologies and instrumentation. The issues associated with this approach are the hands-on time required, and a lack of standardization.
Sample preparation is one of the most critical steps in MS-based proteomic workflows, but the variability of the starting materials (cell/tissue type and protein levels) can be challenging. There is a need for easy-to-use and automatable workflows, along with all-in-one sample preparation solutions, to improve the results' consistency and reliability.
Sample preparation is one of the most critical steps in MS-based proteomic workflows, but the variability of the starting materials (cell/tissue type and protein levels) can be challenging. There is a need for easy-to-use and automatable workflows, along with all-in-one sample preparation solutions, to improve the results' consistency and reliability.
The solution
PreOmics® has developed innovative, flexible, and easy-to-use all-in-one solutions for a number of different sample types, based on its in-StageTip (iST) technology. Pre-cleaning steps, supplied as add-ons, can remove contamination such as detergents. Fractionation steps downstream in the sample preparation process allow in-depth analysis of purified peptides. The sample preparation based on iST technology can be easily automated with PreOmics proprietary liquid handling systems APP96 and PreON, as well as with other third party systems. PreOmics solutions provide researchers with innovations in devices and processes, allowing them to achieve reliable, robust and reproducible sample preparation with increased peptide and protein ID in record time.
Results
Compatible sample types
The iST technology works with sample preparation from plant or mammalian cells and tissues, and with human biological fluids (e.g. plasma). The approach is compatible with chemical labeling (iTRAQ and TMT).
Clinical studies and biomarker discovery
When samples from lung cancer patients and healthy controls were processed with iST and analyzed using mass spectrometry, results showed that the workflow employing our iST technology was able to detect deregulated proteins in cancer cells. This approach has the potential to identify disease biomarkers.
Quantifying host cell protein (HCP) contamination
HCP contamination is one of the biggest challenges when developing biotherapeutics. A fully standardized protocol using our iST technology can meet this challenge when implemented into process control for biotherapeutics production.
"Our researchers happily rely on the PreOmics iST kit knowing that they will have stabilized the sources of technical variations, permitting them to focus on the biology of our precious samples. The PreOmics team has worked closely with our researchers to consult with us on our unique biological questions and concerns"
"Busy MS facilities such as our own rely on efficient, fast, and robust tissue preparation. After a very positive evaluation of the new scalable BeatBox® solution from PreOmics®, we immediately adopted the workflow. Having such a quiet, compact tissue preparation solution on our bench day by day can only further serve to increase our efficiency. Furthermore, BeatBox® allows us to improve our MS facility utilization day by day. Our partnership with PreOmics® remains strong and one where we can exchange ideas and bring long-term outcomes together."
"Busy MS facilities such as our own rely on efficient, fast, and robust tissue preparation. After a very positive evaluation of the new scalable BeatBox® solution from PreOmics®, we immediately adopted the workflow. Having such a quiet, compact tissue preparation solution on our bench day by day can only further serve to increase our efficiency. Furthermore, BeatBox® allows us to improve our MS facility utilization day by day. Our partnership with PreOmics® remains strong and one where we can exchange ideas and bring long-term outcomes together."
Key benefits of the iST technology
Saves time
Sample preparation for MS-based proteomics in a single enclosed device uses a three-step workflow, eliminating or combining stages, in a 2-hour time window.
Suitable for a variety of sample types
Starting material includes prokaryotic and eukaryotic cells, mammalian and plant tissues, and biological fluids (e.g. serum, plasma).
Easy to handle
Our iST kits are simple to use and include all required buffers, reagents and protocols. All researchers need is standard lab equipment.
