Beckman

Smile! Protein and peptide sample preparation automated

Application for high throughput proteomic sample prep for LC-MS analysis with ENRICH-iST on Biomek i7

The challenge:

Achieving consistent, high-quality results in proteomics sample preparation for LC-MS analysis goes beyond addressing common challenges such as sample loss, contamination, and time constraints. The next step is enhancing reproducibility and minimizing manual intervention across large-scale workflows.

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The solution:

Unlock the power of automation. PreOmics® and Beckman® have joined forces to deliver a fully automated, high-throughput LC-MS sample preparation solution. Minimize cross-contamination and significantly enhance protein identification rates by integrating the PreOmics® ENRICH-iST workflow with Beckman®’s Biomek i7 liquid handling platform. The result? High-quality, reproducible data with less hands-on time—ready to meet the demands of high-throughput proteomics.

‍*“Beckman” is a registered Trademark of a third party

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Apply! Simple, streamlined workflows

1. Adding samples

Samples should be liquid, homogenates, or a soft pellet.

2. ENRICH-iST kit setup

Set up the ENRICH-iST consumables on the Beckman Biomek i7 Liquid Handling System®.

3. Sample preparation

Run pre-defined protocols in consultation with your automation specialist.

4. MS analysis

Analyze samples by LC-MS.

5. Data analysis

Process data to see improved peptide identities.

Results

**Figure 1:** Comparison of protein IDs and coefficient of variation (%) from plasma sample preparation following manual and automated workflow. Automation of the ENRICH-iST technology on the Biomek i7 workstation resulted in a higher number of protein identifications and comparable intraplate variation (n = 48 for plasma samples and blanks).
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**Figure 2:** Cross-contamination during plasma sample preparation following manual and automated workflows. The total protein intensity in blanks prepared using the Biomek i7 workstation is negligible compared to plasma samples, indicating no cross-contamination during automation (n = 48 for plasma samples and blanks).

More detailed explanations about these results can be found in the Beckman® application note.

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PreOmics^®'

Beckman

values

Smile

Improved sample quality

Fully automated workflow, from beads preparation to clean peptides
Avoid cross-contamination
High yield in protein identifications

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Relax

Automated high throughput sample preparation

Achieve high reproducibility in plasma sample preparation for LC-MS analysis
Process 1 to 96 samples within 6 hours
Significantly reduce hands-on time

Realize

Cleaner peptides ready for analysis

Enhance throughput, consistency, and data quality
Accelerate biomarker discovery and facilitate large-cohort proteomics studies
Suitable for small- and large-scale studies

PepSep® Columns & Emitters

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Application for high throughput proteomic sample prep for LC-MS analysis with ENRICH-iST on Biomek i7

Beckman

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Instrument

Application for high throughput proteomic sample prep for LC-MS analysis with ENRICH-iST on Biomek i7

Beckman

Request quote

Instrument

Application for high throughput proteomic sample prep for LC-MS analysis with ENRICH-iST on Biomek i7

PepSep® Classics* – Performance Meets Simplicity

Versatile columns for standard and advanced proteomics workflows.

50 cm × 75 µm × 1.5 µm → Spectral Library Generation | Art.-Nr. 1895846
25 cm × 75 µm × 1.5 µm → Sensitivity | Art.-Nr. 1893484
25 cm × 150 µm × 1.5 µm → Versatility | Art.-Nr. 1893476
15 cm × 150 µm × 1.5 µm → Throughput | Art.-Nr. 1893474
8 cm × 150 µm × 1.5 µm → Speed | Art.-Nr. 1893474

* additional column dimensions available. The full list of dimensions can be found in the brochure

PepSep® Advanced – Next Level Sensitivity

For the highest performance and maximum peptide coverage.

25 cm × 75 µm × 1.5 µm → New gold standard | Art.-Nr. 1919826

CaptiveSpray 2 Emitters

Plug-and-play emitters for hassle-free CaptiveSpray setup. Two variants, separate from columns for easy replacement and longer LC-MS system life with peak performance (Pack of 2 pieces).