"Using PepSep Advanced columns on our timsTOF Pro coupled to a nanoElute2, we routinely identify over 5,600 protein groups and 57,000+ unique peptides per run from just 10 ng of HeLa digest — with inter-run CVs for retention time consistently below 1.3% across replicates. The combination delivers a robust, reproducible workflow that we now rely on as the backbone of our clinical proteomics service.”