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Unlock deeper biofluid proteomics for biomarker discovery
From complete, ready-to-use sample preparation kits todedicated CRO services
The challenge
Biofluid proteomics requires both depth and reproducibility
Plasma, serum, CSF, and other complex biofluids contain proteins across an exceptionally wide concentration range. In LC-MS-based proteomics, signals from highly abundant proteins can mask biologically relevant low-abundance proteins - a challenge that becomes even greater in large-cohort studies requiring reproducible and scalable sample preparation. Addressing both aspects is essential for achieving deeper proteome coverage and revealing meaningful biological insights at scale.
The solution
Comprehensive biofluid proteomics workflows from sample to insight
Enrichment technologies provide access to a broader range of proteins within complex biofluids, increasing the visibility of biologically relevant low-abundance proteins. Integrated with the standardized iST technology, enrichment becomes part of a streamlined end-to-end process that combines proteome depth with reproducibility, scalability, and automation readiness. This enables confident analysis across diverse applications, from exploratory research involving complex, high-dynamic-range sample matrices to large-scale cohort studies. Together, ENRICH-iST and P2-iST Plasma give researchers the flexibility to choose the solution that best matches their study objectives, whether the goal is robust large-scale profiling or deeper exploration of the biofluid proteome.
ENRICH-iST – Biofluid sample preparation simplified
ENRICH-iST is a fast, flexible, antibody-free workflow for deeper proteome profiling across plasma1, serum2, CSF3,4,5, saliva6, cell culture media7, and other complex biofluids. Using single paramagnetic bead-based enrichment, it combines enrichment, digestion, and peptide purification to deliver LC-MS-ready peptides from low sample volumes within hours. Compatible with human and non-human mammalian8,9,10 samples, ENRICH-iST supports reproducible, scalable, and automation-ready biomarker and discovery proteomics.
Results
Deeper proteome coverage from minimal sample input
Unlock deeper plasma and serum proteome coverage from just 20 µL of sample input. ENRICH-iST is species- and matrix-independent, achieves proteome depth comparable to depletion strategies, provides complementary biological information, and delivers approximately 2-fold deeper proteome coverage than neat sample preparation workflows.
Complementary insights beyond affinity-based proteomics
ENRICH-iST provides deep, unbiased proteome coverage and complements targeted affinity-based platforms such as Olink. By providing deeper access to the serum proteome, ENRICH-iST can reveal additional biological information that complements targeted protein quantification approaches.
Beyond plasma: Unlock challenging biofluids
ENRICH-iST is designed for researchers working across multiple sample types. The workflow supports challenging matrices such as synovial fluid and delivers deeper proteome coverage without the need for antibody-based depletion.
P2-iST Plasma – Redefining proteomics depth for plasma samples
P2-iST Plasma: combines proprietary P2 nanoparticle enrichment with the robust iST workflow to deliver up to 6-fold more protein identifications than neat-plasma preparation. Optimized specifically for human EDTA plasma11, the fast, standardized, and automation-ready workflow transforms raw plasma into LC-MS-ready peptides within hours. With reproducible throughput of up to 192 samples per day, it supports biomarker discovery in large-cohort studies.
Results
Proteome depth that reveals disease biology
In a colorectal cancer pilot study (6 CRC patients vs. 6 healthy donors), P2-iST Plasma delivered an approximately 6-fold increase in plasma protein group identifications compared with neat plasma. Spike-in experiments with MART, IL-6, and IL-1β confirmed a linear quantitative response across a broad concentration range, from tens of pg/µL up to at least 12.5 ng/µL, supporting its suitability for biomarker-focused proteomics.
Uncovering biomarkers hidden in the plasma proteome
Applied to a biological cohort of P2-iST-enriched plasma samples (n=82) on the sensitive, high-performance PepSep Advanced column, the workflow enabled clear separation of patient groups by principal component analysis. Extracted ion chromatograms further revealed a lung cancer-specific intensity pattern for ITGA11, demonstrating that P2-iST Plasma preserves biologically meaningful, disease-associated signals for biomarker discovery.
In-Depth Characterization of the P2 Enrichment Workflow
Analysis of over 1,700 human EDTA plasma samples from 25+ sites worldwide identified a P2 core proteome of 708 proteins consistently quantified across all samples, without data imputation. This core proteome remains stable across diverse collection methods and is notably enriched for vesicle-associated proteins, including 72 of the top 100 ExoCarta exosome markers — underscoring the robustness of P2 enrichment as a quality benchmark.
Automation-ready for scalable biofluid proteomics
Whether you are processing tens or hundreds of samples, ENRICH-iST and P2-iST Plasma integrate with selected automation platforms to streamline sample preparation, reduce hands-on time, and improve workflow standardization.
Supported Platforms
ENRICH-iST
P2-iST Plasma
KingFisher Flex
KingFisher Apex
Opentrons OT-2
Opentrons Flex
Coming soon
Tecan Fluent with i300
Tecan Resolvex A200*)
Beckman Biomek i7
Agilent Bravo
Hamilton
If your instrument is not listed yet or you have questions around automation of our enrichment kit reach out to: info@preomics.com.
*) Only the peptide clean-up step can be performed on the Tecan Resolvex™ A200. The linked application note describes the iST workflow, but the clean-up procedure is also applicable to ENRICH-iST.
What do our customers say?
"At the Helsinki Proteomics Center, we have benchmarked essentially all major plasma depletion/enrichment strategies on the market. When depth matters, P2-iST consistently comes out on top. For us it has been a game changer – it puts robust, deep plasma proteomics into the hands of any lab without expensive proprietary hardware. The reproducibility and depth we see with P2-iST on our Orbitrap Astral platform makes it ideal for medium scale clinical and translational plasma studies."
Dr. Markku Varjosalo
Research Director and Program Director, Institute of Biotechnology, HiLIFE, Faculty of Medicine, University of Helsinki & Scientific Director, Helsinki Proteomics CenterManager Proteomics, Philip Morris International, Switzerland
“[…] ENRICHplus is changing the way we do plasma proteomics. On Bruker’s timsTOF Pro we could achieve on average 3,800 protein identifications per sample with a throughput of 60 samples per day while only using 20ul of plasma. We never before achieved such depth with a single run of plasma, now enabling us to detect tissue leakage proteins and other low abundant potential biomarkers for cancer and neurodegenerative diseases. […] ENRICHplus is a complete, efficient and game changing platform for deep plasma proteomics."
Roman Fischer
Associate Professor and Head of Discovery Proteomics Facility, University of Oxford
Key benefits of the enrichment technology
Enhanced proteome depth
Streamlined enrichment of low-abundance plasma proteins coupled with iST proteomic sample preparation technology for enhanced plasma proteome depth
Fast and reliable results
A fast, easy-to-use, and standardized protocol for high reproducibility and less hands-on time
Versatile technology
Species-independent and flexible technology compatible with plasma, serum, CSF, and other high dynamic range samples of different mammalian species
Automation friendly
Seamlessly integrated into standard automation platforms for processing up to 192 samples per day
Low input samples
Optimized technique to work with low-input starting material (20-100 μL) for large-scale proteomic studies
Comprehensive proteomics offering: Kit-to-Service
Choose the approach that fits your needs, from complete kits for in-house implementation to dedicated proteomics CRO services.
