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Automate! Sample preparation for mass spectrometry-based proteomics
Standardized, reproducible sample preparation for drug discovery, biomarker analysis, and applied proteomics in biopharma and academic research
The challenge
Sample preparation for LC-MS has many challenges. Manual methods are labor-intensive, unreliable, and prone to lack of reproducibility. Additionally, achieving a robust, reliable, reproducible workflow while starting from a wide range of biologically complex sample matrices is necessary to unlock the full power of proteomics analysis.
The solution
For laboratories using third-party automation systems, PreOmics offers broad compatibility to simplify and standardize proteomics workflows. Our kits seamlessly integrate with liquid-handling platforms from Hamilton, Tecan, Agilent, Opentrons, and Beckman Coulter, enabling efficient and reliable sample preparation for LC-MS analysis.
By collaborating closely with leading automation providers, we deliver flexible, scalable, and platform-agnostic solutions designed to reduce hands-on time to just a few minutes. Our validated automation protocols help ensure consistently high-quality, reproducible sample preparation, supporting robust LC-MS proteomics workflows across diverse laboratory automation environments.
By collaborating closely with leading automation providers, we deliver flexible, scalable, and platform-agnostic solutions designed to reduce hands-on time to just a few minutes. Our validated automation protocols help ensure consistently high-quality, reproducible sample preparation, supporting robust LC-MS proteomics workflows across diverse laboratory automation environments.
Results
Comparable protein identifications with reduced variability
Comparison of manual versus automated ENRICH-iST processing shows that both workflows yield a comparable number of identified proteins, while automation significantly reduces variability. Despite similar peptide and protein IDs (A, B) between manual and Opentrons® OT2-based preparation, the automated workflow achieves lower coefficients of variation (CV) at both the peptide level (C) and protein level (D), demonstrating improved reproducibility and consistency of the automated ENRICH-iST processing.
Reduced cross-contamination compared with manual workflow
Cross-contamination assessment during plasma sample preparation comparing manual handling and an automated workflow. Blank samples processed on the Beckman® Biomek i7 workstation show negligible total protein intensity relative to plasma samples, indicating no cross-contamination during automated preparation (n = 48 plasma samples; n = 48 blanks).
High reproducibility across pooled controls and experimental days
Coefficient of variation (%CV) of protein LFQ intensities for pooled plasma samples and individual experimental days. Violin plots depict intra-day variability for pooled injections (blue) and plasma samples from days 1 to 3 (green), as well as inter-day variability across all days (purple). Median CVs range from ~10-16%, highlighting the robust and reproducible performance of the automated ENRICH-iST workflow on the Tecan Fluent® platform.
Key benefits of iST automation
Automation for all
PreOmics solutions are validated across established liquid-handling platforms, enabling seamless integration, reliable performance, and effortless adoption across diverse automation providers.
Rock-solid results
The combination of automation and optimized PreOmics reagent kits delivers exceptional reproducibility and standardization. Generate high-confidence results while reducing sample preparation complexity and LC-MS run times.
Improved productivity
True automation reduces sample preparation failure rates and increases overall laboratory efficiency. Use your time for what really counts!
Eliminate manual pipetting
Reduce variability associated with manual pipetting to improve data reliability while minimizing the risk of repetitive strain injury (RSI) for laboratory personnel.
