Products/iST

Smile! Proteomics sample preparation simplified

Fast, reliable, standardized methods for sample preparation ready for  mass spectrometry analysis

The challenge:

Complex, multi -step methods of proteomics sample preparation for mass spectrometry analysis have inherent challenges of time, contamination and sample loss. Preparing labelled samples over an extended time period with a complex method leads to variation in results. This challenge is exacerbated with large numbers of samples and small starting amount of input material.

The solution:

PreOmics improved sample technology enables robust and reproducible sample preparation. The iST method provides significant time savings as complex workflows previously requiring many different reagents taking 48 hours are replaced by a single kit containing all standardized reagents. The iST kit allows proteomics sample preparation for mass spectrometry analysis, whether manually, semi or fully automated, in a 2 hour time window.

Apply! Simple, streamlined workflows

1.
Sample
Samples should be liquid, homogenates or a soft pellet.
2.
Lyse, reduce, alkylate
Add LYSE reagent, incubate for 10 mins at 95°C.
3.
Digest
Cool, add DIGEST reagent, incubate for 60 mins at 37°C.
4.
Purify
Load onto cartridge, WASH and ELUTE. Dry and resuspend in LOAD.
5.
MS analysis
Inject and analyse to see improved peptide identities.
For technical data and performance statistics see the iST Application note

Time flies with PreOmics iST sample preparation

While standard proteomics sample preparation takes around 44 hours – the same time as flying around the world non-stop, PreOmics kits take the same time as flying from our Munich HQ to London or from Boston to Chicago.  

Increase your sample throughput, improve quality and produce reliable data. 

It’s as easy as i-S-T!
The products displayed are for visual representation only and may differ from those received

iST

iST 8x
P.O.00001
8 sample kit
315 €
iST 96x
P.O.00027
96 sample kit
2,660 €
Racked into 96 well plate, has collars for individual processing
iST HT 192x
P.O.00067
192 sample kit
5,300 €
Reagents in amber bottles for easy use with liquid handlers
iST PreON 8x
P.O.00077
8 sample kit
315 €
With PreON plasticware included
iST PreON 96x
P.O.00079
96 sample kit
2,660 €
With PreON plasticware included

PreOmics iST Values

Smile

Improved sample quality

High reproducibility: R
2
>0.9
Working range: 1-100 µg
Increased peptide and protein  identifications

Relax

Quicker methodology

1 hour hands-on time, 2 hours total time
Single step incorporating lysis, reduction and alkylation
All-in-one solution, all reagents provided
Compatible with standard lab equipment

Realise

Cleaner peptides ready for analysis

Dual clean up step removes hydrophilic and hydrophobic contaminants
Achieve ready to measure peptides
Reduce mass spectrometry downtime
Manual to fully automated processing options available

Imagine

Applying the science
Find out more about the wide range of applications and sample types to which PreOmics technology has been successfully applied