Fast, reliable, standardized methods for sample preparation ready for mass spectrometry analysis
Complex, multi-step methods of proteomics sample preparation for mass spectrometry analysis have inherent challenges of time, contamination and sample loss. Preparing labelled samples over an extended time period with a complex method leads to variation in results. This challenge is exacerbated with large numbers of samples and small starting amount of input material.
PreOmics improved sample technology enables robust and reproducible sample preparation. The iST method provides significant time savings as complex workflows previously requiring many different reagents taking 48 hours are replaced by a single kit containing all standardized reagents. The iST kit allows proteomics sample preparation for mass spectrometry analysis, whether manually, semi or fully automated on the PreON, in a 2 hour time window.
Apply! Simple, streamlined workflows
Samples should be liquid, homogenates or a soft pellet.
2. Lyse, reduce, alkylate
Add LYSE reagent, incubate for 10 mins at 95°C.
Cool, add DIGEST reagent, incubate for 60 mins at 37°C.
Load onto cartridge, WASH and ELUTE. Dry and resuspend in LOAD.
5. MS analysis
Inject and analyse to see improved peptide identities.
For technical data and performance statistics see the iST Application note
While standard proteomics sample preparation takes around 44 hours – the same time as flying around the world non-stop, PreOmics kits take the same time as flying from our Munich HQ to London or from Boston to Chicago.
Increase your sample throughput, improve quality and produce reliable data.
It’s as easy as i-S-T!
PreOmics iST Values
Improved sample quality
High reproducibility: R
Working range: 1-100 µg
Increased peptide and protein identifications
Shipped at RT🌿
1 hour hands-on time, 2 hours total time
Single step incorporating lysis, reduction and alkylation
All-in-one solution, all reagents provided
Compatible with standard lab equipment
Cleaner peptides ready for analysis
Dual clean up step removes hydrophilic and hydrophobic contaminants
Achieve ready to measure peptides
Reduce mass spectrometry downtime
Manual to fully automated processing options available
The products displayed are for visual representation only and may differ from those received
8 sample kit
96 sample kit
Racked into 96 well plate, has collars for individual processing
iST HT 192x
192 sample kit
Reagents in amber bottles for easy use with liquid handlers