Smile! Immunoprecipitation sample preparation simplified

Fast, reliable, preparation from immunoprecipitated samples ready for mass spectrometry

The challenge:

Integrating immunoprecipitation with multi-step methods of proteomics sample preparation for mass spectrometry analysis has inherent challenges of time, contamination and sample loss. Preparing labelled samples over extended period with a complex method leads to variation in results especially when working with high sample throughput and small starting amounts of input material.

The solution:

The PreOmics / ChromoTek iST-Trap Kit enables the fast and efficient immunoprecipitation of GFP-Fusion proteins and the interaction factors followed by the innovative sample preparation for mass spectrometry based proteomics. All in less than 4 hours.

Apply! Simple, streamline workflows

1. Sample immunoprecipitation
Samples should be liquid, add GFP-Trap_A and incubate at 4°C for 60 min.
2. Denature, reduce and alkylate
Add LYSE reagent, and incubate for 10 mins at 60°C.
3. Digest
Cool, add DIGEST reagent, incubate for 60 mins at 37°C.
4. Purify
Load onto cartridge, WASH and ELUTE. Dry and resuspend in LOAD.
5. MS analysis
Inject and analyze to see improved peptide identities.

Results

Time flies with PreOmics iST-Trap sample preparation

While standard proteomics sample preparation takes up to 44 hours – the same time as flying around the world non-stop, PreOmics kits take the same time as flying from our Munich HQ to London or from Boston to Chicago. Increase your sample throughput, improve quality and produce reliable data. It’s as easy as i-S-T!

PreOmics'

iST-Trap

values

Smile

Improved immunoprecipitation sample quality
  • Reliable identification of GFP-fusion proteins and interactomes
  • Working range: 1-100 µg
  • Increased peptide and protein identifications
  • Shipped at RT🌿

Relax

Quicker methodology
  • Less than 4 hours hands-on time
  • Single step incorporating lysis, reducation and alkylation
  • All-in-one solution, all reagents provided
  • Compatible with standard lab equipment

Realize

Cleaner peptides ready for analysis
  • Dual clean up step removes hydrophilic and hydrophobic contaminants
  • Achieve ready-to-measure peptides
  • Reduce mass spectrometry downtime
  • Range of processing options

iST-Trap

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Instrument
Instrument
Fast, reliable, preparation from immunoprecipitated samples ready for mass spectrometry
Instrument
Instrument
Fast, reliable, preparation from immunoprecipitated samples ready for mass spectrometry
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Applying the science
Find out more about the wide range of applications and sample types to which PreOmics technology has been successfully applied