Smile! High throughput sample preparation simplified
Fast, reliable, standardized methods for bottom-up proteomic sample preparation on positive pressure devices
As LC-MS instrumentation has developed, there is a growing need to increase sample throughput and sample preparation can become the rate-limiting step.
The PreOmics iST-PSI workflow couples the simplicity of the iST technology with positive pressure plate processing. Positive pressure processing is known to improve recovery and reproducibility compared to negative pressure or centrifugation. The format is easily automated on major liquid handling platforms.
Apply! Simple, streamlined workflows
Samples should be liquid, homogenates or a soft pellet.
2. Lyse, reduce, alkylate
Add LYSE reagent, incubate for 10 mins at 95°C.
Cool, add DIGEST reagent, incubate for 60 mins at 37°C.
4. Processing on positive pressure device
Load onto SPE plate. WASH and ELUTE on positive pressure device. Dry and resuspend in LOAD.
5. MS analysis
Inject and analyze to see improved peptide identities.
For technical data and performance statistics see the Hamilton® application note below.
“Hamilton” is a registered Trademark of a third party
Time flies with PreOmics iST-PSI sample preparation
While standard proteomics sample preparation takes around 44 hours – the same time as flying around the world non-stop, PreOmics kits take the same time as flying from our Munich HQ to London or from Boston to Chicago.
Increase your sample throughput, improve quality and produce reliable data.
It’s as easy as i-S-T!
The products displayed are for visual representation only and may differ from those received
iST-REG-PSI 96HT (192rxn)
192 sample kit
3.800 € | 4.560 $
Complete iST sample preparation with 2 clean-up runs with 96 samples each on positive pressure devices
iST-REG-PSI Buffer Add-on
380 € | 460 $
Buffers in amber bottles for additional clean-up runs
PreOmics iST-PSI Values
Improved sample quality
High reproducibility: Pearson correlation > 0.93
Working range: 1-100 µg
Increased peptide and protein identifications
High throughput sample preparation
High throughput sample preparation: 96 samples within 3 hours
Applications notes available for popular liquid handling platforms
Can be used on manual positive pressure manifolds
Cleaner peptides ready for analysis
Dual clean up step removes hydrophilic and hydrophobic contaminants
Achieve ready-to-measure peptides
Reduce mass spectrometry downtime
Gain greater benefit from your installed automation platform
Applying the science
Find out more about the wide range of applications and sample types to which PreOmics technology has been successfully applied