Fast, reliable, reproducible sample preparation for chemical labeling (iTRAQ and TMT)
Chemical labeling reagents have specific handling and compatibility requirements to achieve good labeling efficiencies. Combined with the multi-step methods of proteomics sample preparation for mass spectrometry analysis, chemical labelling protocols have inherent challenges of time, contamination and sample loss. This challenge is exacerbated with large numbers of samples and small starting amount of input material.
PreOmics iST-NHS sample preparation is fully compatible with chemical labeling reagents. Allowing streamlined peptide processing, chemical labeling and an efficient peptide clean-up step. The iST-NHS method decreases hands-on time, sample loss and the amount of chemical label required. Complex workflows previously requiring many different reagents over a two day time period are replaced by a single kit containing all standardized reagents to accomplish proteomics sample labeling and preparation for mass spectrometry protein analysis whether manually, semi or fully automated on the PreON in a 2.5 hour time window.
Apply! Simple, streamlined workflows
Samples should be liquid, homogenates or a soft pellet.
2. Lyse, reduce, alkylate
Add LYSE reagent, incubate for 10 mins at 95°C.
3. Digest and label
Cool, add DIGEST reagent, incubate for 60 mins at 37°C. Label according to vendor instructions.
Load onto cartridge, WASH and ELUTE. Dry and resuspend in LOAD.
5. MS analysis
Inject and analyse to see improved peptide identities.
For technical data and performance statistics see the iST-NHS Application note.
While standard proteomics sample preparation takes around 44 hours – the same time as flying around the world non-stop, PreOmics kits take the same time as flying from our Munich HQ to London or from Boston to Chicago.
Increase your sample throughput, improve quality and produce reliable data.
It’s as easy as i-S-T!
PreOmics iST-NHS Values
Improved sample quality
High reproducibility: R
Working range: 1-100 µg
Increased peptide and protein identifications
Shipped at RT🌿
2.5 hour total time, including labeling
Single step incorporating lysis, reduction and alkylation
All-in-one solution, all reagents provided
Compatible with standard lab equipment
Cleaner peptides ready for analysis
Dual clean up step removes hydrophilic and hydrophobic contaminants
Achieve ready-to-measure labeled peptides
Reduce mass spectrometry downtime
Manual to fully automated processing options available
The products displayed are for visual representation only and may differ from those received
12 sample kit
96 sample kit
Racked in 96 well plate, has collars for individual processing
iST-NHS HT 192x
192 sample kit
Reagents in amber bottles for easy use with liquid handlers