Products/iST-NHS

Smile! Chemically labeled sample preparation simplified

Fast, reliable, reproducible sample preparation for chemical labeling (iTRAQ and TMT)

The challenge:

Chemical labeling reagents have specific handling and compatibility requirements to achieve good labeling efficiencies. Combined with the multi -step methods of proteomics sample preparation for mass spectrometry analysis, chemical labelling protocols have inherent challenges of time, contamination and sample loss.  This challenge is exacerbated with large numbers of samples and small starting amount of input material.

The solution:

PreOmics iST-NHS sample preparation is fully compatible with chemical labeling reagents. Allowing streamlined peptide processing, chemical labeling and an efficient peptide clean-up step. The iST-NHS method decreases hands-on time, sample loss and the amount of chemical label required. Complex workflows previously requiring many different reagents over a two day time period are replaced by a single kit containing all standardized reagents to accomplish proteomics sample labeling and preparation for mass spectrometry  protein analysis whether manually, semi or fully automated on the PreON in a 2.5  hour time window.

Apply! Simple, streamlined workflows

1.
Samples
Samples should be liquid, homogenates or a soft pellet.
2.
Lyse, reduce, alkylate
Add LYSE reagent, incubate for 10 mins at 95°C.
3.
Digest and label
Cool, add DIGEST reagent, incubate for 60 mins at 37°C. Label according to vendor instructions.
4.
Purify
Load onto cartridge, WASH and ELUTE. Dry and resuspend in LOAD.
5.
MS analysis
Inject and analyse to see improved peptide identities.
For technical data and performance statistics see the iST-NHS Application note.

Time flies with PreOmics iST sample preparation

While standard proteomics sample preparation takes around 44 hours – the same time as flying around the world non-stop, PreOmics kits take the same time as flying from our Munich HQ to London or from Boston to Chicago.  

Increase your sample throughput, improve quality and produce reliable data. 

It’s as easy as i-S-T!
The products displayed are for visual representation only and may differ from those received

iST-NHS

iST-NHS 12x
P.O.00026
12 sample kit
360 €
iST-NHS 96x
P.O.00030
96 sample kit
1,900 €
Racked in 96 well plate, has collars for individual processing
iST-NHS HT 192x
P.O.00083
192 sample kit
3,800 €
Reagents in amber bottles for easy use with liquid handlers
iST-NHS PreON 12x
P.O.00078
12 sample kit
465 €
With PreON plasticware included
iST-NHS PreON 96x
P.O.00080
96 sample kit
2,300 €
With PreON plasticware included

PreOmics iST-NHS Values

Smile

Improved sample quality

High reproducibility: R
2
>0.9
Working range: 1-100 µg
Increased peptide and protein  identifications

Relax

Quicker methodology

2.5 hour total time, including labeling
Single step incorporating lysis, reduction and alkylation
All-in-one solution, all reagents provided
Compatible with standard lab equipment

Realise

Cleaner peptides ready for analysis

Dual clean up step removes hydrophilic and hydrophobic contaminants
Achieve ready-to-measure labeled peptides
Reduce mass spectrometry downtime
Manual to fully automated processing options available

Imagine

Applying the science
Find out more about the wide range of applications and sample
types to which PreOmics technology has been successfully applied