Explore these publications to discover how iST-based proteomics sample preparation supports targeted protein degradation (TPD) research.
DCAF1 PROTACs mediate the degradation of tyrosine kinases localized in the plasma membrane, cytosol as well as nucleoplasm. Proteomic profiling of HEK293T and TMD8 cells treated with DDa-1 and DCAF1 binders confirmed downregulation of CSK and LIMK2 kinases and resulted in discovery of two new non-receptor tyrosine kinases (TEC and TNK2). Cells treated with the different degraders were prepared for the TMT-based quantitative profiling using the iST-NHS kit from PreOmics®.
Read the publicationCereblon (CRBN) selectivity is re-directed by glue degrader NVP-DKY709 in Treg cells. Deficiency of IKZF2 (Helios) transcription factor reduces tumor growth in mice. Quantitative proteomics profiling of Jurkat T cells treated for 16 h with NVP-DKY709 (or DMSO as control) revealed significant downregulation of IKZF2. Cell samples for this approach were prepared using the iST-NHS kit from PreOmics® on the PreON automation platform.
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