Reproducible and reliable tissue homogenization needs to produce a smooth and easy to pipette homogenate and minimize the possibility of cross-contamination. A specific consideration for proteomics is the release of the maximum number of proteins to make them accessible for the next steps of sample preparation. Additionally, lab space, the requirement for chiller units, and the noise made by some processes can also impact instrument choice.
The solution:
The BeatBox is a fast and easy to use instrument for up to 96 samples, which completes the homogenization in as little as 10 minutes without sample cross contamination and minimal heat induction. The BeatBox has a surprisingly small footprint and quiet operation in comparison to traditional tissue processing machines. With the BeatBox, tissue homogenization can be seamlessly integrated into the PreOmics iST sample preparation workflows.
Relax! iST compatible workflows
1. Preparing samples
Cut tissue into appropriate size or volume of cells
2. BeatBox Tissue add-on kit
Add your tissue or cells and the LYSE buffer from iST kit family
3. BeatBox your samples
Select the power and time, then press START
4. Digest and clean-up
Continue with step 2 from selected iST workflow
5 Mass spectrometric analysis
Inject and analyze your samples
Results
BeatBox homogenates show the highest protein yields Reproducibility of protein yields from different tissues were increased by 2.5x using BeatBox.
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