Samples containing biopharmaceutical proteins and peptides can have issues with efficient preparation of peptides for analysis. For example monoclonal antibodies have a high number of disulfide bridges and need optimized reduction and alkylation steps. The multi-step methods of protein sample preparation for mass spectrometry analysis have inherent challenges of time, contamination and sample loss.

PreOmics improved sample technology,  iST-BCT sample preparation kit was developed to enable the robust processing of biological fluids, HCPs and therapeutic proteins such as monoclonal antibodies. iST-BCT has been optimized to minimize artificial modifications (e.g. deamidation, oxidation) and increase the alkylation rate. Complex workflows previously requiring many different reagents over a two day time period are replaced by a single kit containing all standardized reagents to accomplish preparation for mass spectrometry  protein analysis whether manually, semi or fully automated on the PreON in a 2 hour time window.