The multi-step methods of protein sample preparation for mass spectrometry analysis have inherent challenges of time, contamination and sample loss. When preparing biological fluids (e.g. plasma and serum) for proteomic analyses, harsh reagents may introduce artificial modifications resulting in reduced protein and peptide identifications.
PreOmics’ iST-BCT sample preparation kit was developed to enable the robust processing of biological fluids and has been optimized to minimize artificial modifications (e.g. deamidation, oxidation) and increase the alkylation rate. Complex workflows previously requiring many different reagents over a two day time period are replaced by a single kit containing all standardized reagents to accomplish preparation for mass spectrometry protein analysis whether manually, semi or fully automated on the PreON in a 2 hour time window.
Apply! Simple, streamlined workflows
Samples should be liquid, homogenates or a soft pellet.
2. Lyse, reduce, alkylate
Add LYSE reagent, incubate for 10 mins at 95°C.
Cool, add DIGEST reagent, incubate for 60 mins at 37°C.
Load onto cartridge, WASH and ELUTE. Dry and resuspend in LOAD.
5. MS analysis
Inject and analyse to see improved peptide identities.
For technical data and performance statistics see the iST-BCT Application Note and the Bruker Flashnote here
While standard proteomics sample preparation takes around 44 hours – the same time as flying around the world non-stop, PreOmics kits take the same time as flying from our Munich HQ to London or from Boston to Chicago.
Increase your sample throughput, improve quality and produce reliable data.
It’s as easy as i-S-T!
PreOmics iST-BCT Values
Improved sample quality
High reproducibility: R
Working range: 1-100 µg
Increased peptide and protein identifications
Shipped at RT🌿
1 hour hands-on time
Single step incorporating lysis, enhanced reduction and alkylation
All-in-one solution, all reagents provided
Compatible with standard lab equipment
Cleaner peptides ready for analysis
Dual clean up step removes hydrophilic and hydrophobic contaminants
Achieve ready-to-measure peptides
Reduce mass spectrometry downtime
Manual to fully automated processing options available
The products displayed are for visual representation only and may differ from those received
8 sample kit
96 sample kit
Racked into 96 well plate, has collars for individual processing