Products/iST-BCT

Smile! Biological fluid sample preparation simplified

Fast, reliable, reproducible sample preparation minimizing artificial modifications

The challenge:

The multi-step methods of protein sample preparation for mass spectrometry analysis have inherent challenges of time, contamination and sample loss. When preparing biological fluids (e.g. plasma and serum) for proteomic analyses, harsh reagents may introduce artificial modifications resulting in reduced protein and peptide identifications.

The solution:

PreOmics’ iST-BCT sample preparation kit was developed to enable the robust processing of biological fluids and has been optimized to minimize artificial modifications (e.g. deamidation, oxidation) and increase the alkylation rate. Complex workflows previously requiring many different reagents over a two day time period are replaced by a single kit containing all standardized reagents to accomplish preparation for mass spectrometry protein analysis whether manually, semi or fully automated on the PreON in a 2 hour time window.

Apply! Simple, streamlined workflows

1.
Samples

Samples should be liquid, homogenates or a soft pellet.

2.
Lyse, reduce, alkylate

Add LYSE reagent, incubate for 10 mins at 95°C.

3.
Digest

Cool, add DIGEST reagent, incubate for 60 mins at 37°C.

4.
Purify

Load onto cartridge, WASH and ELUTE. Dry and resuspend in LOAD.

5.
MS analysis

Inject and analyse to see improved peptide identities.

For technical data and performance statistics see the iST-BCT Application Note and the Bruker Flashnote here

iST-BCT | Same Day HCP Workflow Has Become Reality | Bruker Flashnote

Time flies with PreOmics iST sample preparation

While standard proteomics sample preparation takes around 44 hours – the same time as flying around the world non-stop, PreOmics kits take the same time as flying from our Munich HQ to London or from Boston to Chicago.

Increase your sample throughput, improve quality and produce reliable data.

It’s as easy as i-S-T!