Fast, reliable, reproducible sample preparation of biological fluids and therapeutic proteins e.g. mABs
Samples containing biopharmaceutical proteins and peptides can have issues with efficient preparation of peptides for analysis. For example monoclonal antibodies have a high number of disulfide bridges and need optimized reduction and alkylation steps. The multi-step methods of protein sample preparation for mass spectrometry analysis have inherent challenges of time, contamination and sample loss.
PreOmics improved sample technology, iST-BCT sample preparation kit was developed to enable the robust processing of biological fluids, HCPs and therapeutic proteins such as monoclonal antibodies. iST-BCT has been optimized to minimize artificial modifications (e.g. deamidation, oxidation) and increase the alkylation rate. Complex workflows previously requiring many different reagents over a two day time period are replaced by a single kit containing all standardized reagents to accomplish preparation for mass spectrometry protein analysis whether manually, semi or fully automated on the PreON in a 2 hour time window.
Apply! Simple, streamlined workflows
Samples should be liquid, homogenates or a soft pellet.
2. Lyse, reduce, alkylate
Add LYSE reagent, incubate for 10 mins at 95°C.
Cool, add DIGEST reagent, incubate for 60 mins at 37°C.
Load onto cartridge, WASH and ELUTE. Dry and resuspend in LOAD.
5. MS analysis
Inject and analyse to see improved peptide identities.
For technical data and performance statistics see the iST-BCT Application Note and the Bruker Flashnote here
While standard proteomics sample preparation takes around 44 hours – the same time as flying around the world non-stop, PreOmics kits take the same time as flying from our Munich HQ to London or from Boston to Chicago.
Increase your sample throughput, improve quality and produce reliable data.
It’s as easy as i-S-T!
The products displayed are for visual representation only and may differ from those received
8 sample kit
96 sample kit
Racked into 96 well plate, has collars for individual processing
PreOmics iST-BCT Values
Improved sample quality
High reproducibility: R
Working range: 1-100 µg
Increased peptide and protein identifications
1 hour hands-on time
Single step incorporating lysis, enhanced reduction and alkylation
All-in-one solution, all reagents provided
Compatible with standard lab equipment
Cleaner peptides ready for analysis
Dual clean up step removes hydrophilic and hydrophobic contaminants
Achieve ready-to-measure peptides
Reduce mass spectrometry downtime
Manual to fully automated processing options available
Applying the science
Find out more about the wide range of applications and sample types to which PreOmics technology has been successfully applied