Additional data can be found in the Tecan® SP3-iST application note
. The numbers of PSMs and IDs for both peptides and proteins were very consistent for all samples, regardless of protein input, as well as between the manual and the automated protocols. Approximately 27,370 peptides and 2,960 proteins were identified in all samples, in agreement with the expected performance of the nanoLC-MS measurements conducted. This demonstrates the high efficiency, quality and robustness of the automated SP3-iST workflow for the entire range of tested input amounts (15-50 μg).