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Smile! High throughput sample preparation simplified
Fast, reliable, standardized methods for bottom-up proteomic sample preparation on positive pressure devices
The challenge:
As LC-MS instrumentation has developed, there is a growing need to increase sample throughput and sample preparation can become the rate-limiting step.
The challenge:
The PreOmics® iST-PSI workflow couples the simplicity of the iST technology with positive pressure plate processing. Positive pressure processing is known to improve recovery and reproducibility compared to negative pressure or centrifugation. The format is easily automated on major liquid handling platforms including Tecan Resolvex A200 and Hamilton [MP]2.
Apply! Simple, streamlined workflows
1. Sample
Samples should be liquid, homogenates, or soft pellets.
2. Lyse, reduce, alkylate
Add LYSE reagent, and incubate for 10 mins at 95°C.
3. Digest
Cool, add DIGEST reagent, and incubate for 60 mins at 37°C.
4. Processing on positive pressure device
Load onto SPE plate. WASH and ELUTE on a positive pressure device. Dry and resuspend in LOAD.
5. MS analysis
Inject and analyze to see improved peptide identities.
Results
Time flies with PreOmics® iST-PSI sample preparation
While standard proteomics sample preparation takes up to 44 hours – the same time as flying around the world non-stop, PreOmics® kits take the same time as flying from our Munich HQ to London or from Boston to Chicago. Increase your sample throughput, improve quality and produce reliable data. It’s as easy as i-S-T!
PreOmics®'
iST-PSI
values
Smile
Improved sample quality
- High reproducibility: Pearson correlation > 0.93
- Working range: 1-100 µg
- Increased peptide and protein identifications
- Shipped at RT🌿
Relax
High throughput sample preparation
- High throughput sample preparation: 96 samples within 3 hours
- Applications notes available for popular liquid handling platforms
- Can be used on manual positive pressure manifolds
Realize
Cleaner peptides ready for analysis
- Dual clean up step removes hydrophilic and hydrophobic contaminants
- Achieve ready-to-measure peptides
- Reduce mass spectrometry downtime
- Gain greater benefit from your installed automation platform
iST-PSI
iST-PSI
Request quote
Instrument
Instrument
Fast, reliable, standardized methods for bottom-up proteomic sample preparation on positive pressure devices
iST-PSI
Request quote
Instrument
Instrument
Fast, reliable, standardized methods for bottom-up proteomic sample preparation on positive pressure devices
iST-PSI
Request quote
Instrument
Instrument
Fast, reliable, standardized methods for bottom-up proteomic sample preparation on positive pressure devices
Request quote
iST-REG-PSI 96HT (192rxn)
P.O.00108
192-sample kit
$5,592
4.659 €
Complete iST sample preparation with 2 clean-up runs with 96 samples each on positive pressure devices
iST-REG-PSI Buffer Add-on
P.O.00109
Buffer Add-on
$617
509 €
Buffers in amber bottles for additional clean-up runs
The PreOmics® products displayed are for visual representation only and may differ from those received
iST-PSI | Automation of the iST-PSI PreOmics workflow on Tecan`s Freedom EVO® 100 and Resolvex® A200 | Tecan
iST-PSI
TECAN
iST-PSI | Automation of the iST-PSI PreOmics workflow on Tecan`s Freedom EVO® 100 and Resolvex® A200 | Tecan
iST-PSI | Automation of the iST-PSI PreOmics workflow on Tecan`s Freedom EVO® 100 and Resolvex® A200 | Tecan
iST-PSI
TECAN
iST-PSI | Automation of the iST-PSI PreOmics workflow on Tecan`s Freedom EVO® 100 and Resolvex® A200 | Tecan
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2:34
min
May 17, 2021
All you need to know about the iST-PSI kit
Fast, reliable, standardized method for bottom-up proteomic sample preparation on positive pressure devices
Watch video
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