Products/iST-PSI

Smile! High throughput sample preparation simplified

Fast, reliable, standardized methods for bottom-up proteomic sample preparation on positive pressure devices

The challenge:

As LC-MS instrumentation has developed, there is a growing need to increase sample throughput and sample preparation can become the rate-limiting step.

The solution:

The PreOmics iST-PSI workflow couples the simplicity of the iST technology with positive pressure plate processing. Positive pressure processing is known to improve recovery and reproducibility compared to negative pressure or centrifugation. The format is easily automated on major liquid handling platforms.

Apply! Simple, streamlined workflows

1.
Sample

Samples should be liquid, homogenates or a soft pellet.

2.
Lyse, reduce, alkylate

Add LYSE reagent, incubate for 10 mins at 95°C.

3.
Digest

Cool, add DIGEST reagent, incubate for 60 mins at 37°C.

4.
Processing on positive pressure device

Load onto SPE plate. WASH and ELUTE on positive pressure device. Dry and resuspend in LOAD.

5.
MS analysis

Inject and analyze to see improved peptide identities.

For technical data and performance statistics see the Hamilton® application note below.

“Hamilton” is a registered Trademark of a third party

High-Throughput Automation of the PreOmics iST Technology for Proteomics LC-MS Sample Preparation | Hamilton

Time flies with PreOmics iST-PSI sample preparation

While standard proteomics sample preparation takes around 44 hours – the same time as flying around the world non-stop, PreOmics kits take the same time as flying from our Munich HQ to London or from Boston to Chicago.

Increase your sample throughput, improve quality and produce reliable data.

It’s as easy as i-S-T!