Common mass spectrometry (MS)-based proteomic workflows are multistep processes employing different methodologies and instrumentation. The issues associated with this approach are the hands-on time required, and a lack of standardization.
Sample preparation is one of the most critical steps in MS-based proteomic workflows, but the variability of the starting materials (cell/tissue type and protein levels) can be challenging. There is a need for easy-to-use and automatable workflows, along with all-in-one sample preparation solutions, to improve the results' consistency and reliability.
PreOmics has developed innovative, flexible, and easy-to-use all-in-one solutions for a number of different sample types, based on its in-StageTip (iST) technology. Pre-cleaning steps, supplied as add-ons, can remove contamination such as detergents. Fractionation steps downstream in the sample preparation process allow in-depth analysis of purified peptides. The iST technology provides researchers with innovations in devices and processes, allowing them to achieve reliable, robust and reproducible sample preparation with increased peptide and protein ID in record time.
When samples from lung cancer patients and healthy controls were processed with iST and analyzed using mass spectrometry, results showed that the workflow employing our iST technology was able to detect deregulated proteins in cancer cells. This approach has the potential to identify disease biomarkers.
"Our researchers happily rely on the PreOmics iST kit knowing that they will have stabilized the sources of technical variations, permitting them to focus on the biology of our precious samples. The PreOmics team has worked closely with our researchers to consult with us on our unique biological questions and concerns"
Sample preparation for MS-based proteomics in a single enclosed device uses a three-step workflow, eliminating or combining stages, in a 2-hour time window.
Starting material includes prokaryotic and eukaryotic cells, mammalian and plant tissues, and biological fluids (e.g. serum, plasma).
Our iST kits are simple to use and include all required buffers, reagents and protocols. All researchers need is standard lab equipment.
Want to further increase reproducibility and standardization, while reducing hands-on time (down to five minutes) and human error? Relax and let our PreON automation platform do the work for you.
Streamline your proteomics sample preparation: Our iST kit replaces complex workflows with a single kit containing all standardized reagents while providing significant time savings
What do our award-winning iST kits look like and what exactly do they do? Watch this short video for a quick primer on the gold standard of simple, robust sample preparation for LCMS analysis
Unique LC-MS workflow for accurate quantification and detection of individual host cell proteins
The ENRICH-iST workflow provides a fast, easy-to-use, standardized, and automatable protocol for high-throughput proteome profiling of blood-derived biofluidic samples
Efficient and simplified processing of various mammalian tissues using the iST technology for reproducible and sensitive proteomic analyses
Standardized, high-throughput platform for automated, rapid, and extensive plasma proteome characterization
In-Stage Tip Digestion improves sample preparation prior to LC-MS/MS analysis for clinical research