Applications/Sample types/Tissue-FFPE

Plan! Use PreOmics' products for your research

Resources for working with fresh and FFPE tissue samples
Cell lines
SELECT
Model Organisms
SELECT
Tissue/FFPE
Plant
SELECT
Biological Fluids
SELECT
Immunoprecipitation
SELECT

Tissue/FFPE

Tissues may be the starting point for a wide range of experimental investigations from discovery protein interaction studies, pharmacodynamic effects, through to large clinical trial biomarker measurements. For example looking at differential protein expression across a tissue section across a tumour, either from a recent biopsy or from a biobank FFPE slide. Homogenised tissues are compatible for processing with our iST and iST-NHS kits. .

The iST/iST-NHS kits are suitable for use in research and regulated environments. The kits contain all you need for your peptide or protein sample preparation and suitable for use by all members of your lab, no specialist experience is needed.

The flexible formats of single samples or racked in to 96 well holders allow the kits to be used manually or with a range of automation options. The cartridges can be processed by centrifugation or using positive pressure manifolds, again giving options for all labs. Dedicated resources are shown below and our technical support team would be happy to help with advice.

Please always ensure the total protein present is within the 1 – 100 µg range. Guidelines are given below dependent on the starting sample type.

Mammalian Tissue:

Mammalian tissue samples are more difficult to lyse and require some kind of mechanical force disruption, For example the Diagenode Bioruptor Pico, in the presence of our LYSE or LYSE-NHS buffers . We also recommend addition of glass beads in combination with ultra sonication for most efficient tissue homogenization and cell lysis

FFPE Tissue:

Processing of FFPE samples requires de-paraffinization of the FFPE punches/slides. In addition, de-paraffinized samples require a longer heat treatment for efficient de-crosslinking, increase the initial heating step from 10 to 60 min at 95°C.

PreOmics Values

Smile

Improved sample quality

High reproducibility: R
2
>0.9
Working range: 1-100 µg
Increased peptide and protein identifications

Relax

Quicker methodology

Less than 5 hours for complete workflow
Single step incorporating lysis, reduction and alkylation
All-in-one solution, all reagents provided
Compatible with standard lab equipment

Realise

Cleaner peptides ready for analysis

Dual clean up step removes hydrophilic and hydrophobic contaminants
Achieve ready-to-measure peptides
Reduce mass spectrometry downtime
Manual to fully automated processing options available

Imagine

Applying the science
Find out more about the wide range of applications and sample
types to which PreOmics technology has been successfully applied