In targeted mass spectrometry-based protein quantification, information about the peptide or protein is already known, typical experiments include:
· Protein dynamics e.g. steady-state concentration, turn-over rates (PK, PD studies)
· Absolute quantitation e.g. biomarker measurement or dosed biological drugs
· Relative quantitation
Consideration of appropriate internal standards in needed, stable-isotope labelled proteins should be added before the digestion step and stable-isotope labelled peptides can be spiked-into our iST and iST-NHS workflows after the digestion step. Additionally surrogate matrices maybe needed for calibration and quality control samples. Dedicated resources are shown below and our technical support team would be happy to help with advice.