Research and discovery covers a wide range of experimental designs and investigations including:
● Bottom-up proteomics for changes in protein expression e.g. disease versus healthy or dosed versus placebo
● Biological mechanism understanding:
o Metabolic pathway elucidation
o Protein functionality pathway where one or more genes are silenced in one specific condition and global proteomic analysis is performed to compare silencing sample to a control/wild type sample
o Protein interaction studies e.g.protein : protein or protein : drug
o Protein localisation e.g. identification of cellular compartment or across a tissue slice tumour or healthy
Our iST kits are suitable for experiments with metabolic labeling or in label-free mode, whereas our iST-NHS kits are suitable for experiments with chemical labeling such as iTRAQ or TMT. Protocols are also available where immunoprecipitation is used for enrichment. Dedicated resources are shown below and our technical support team is would be happy to help with advice.