Products/Phospho

Smile! Phosphoproteomics sample preparation simplified

Fast, reliable, reproducible sample preparation for phosphoproteomics workflows

The challenge:

Phosphoproteomics sample preparation methods for mass spectrometry analysis have inherent challenges of time, contamination and sample loss. Preparing labelled samples over extended period with a complex method leads to variation in results. This challenge is exacerbated when high starting protein concentrations are needed to acheive detection limits and when in combination with enrichment techniques and with sample throughput requirements.

The solution:

PreOmics improved sample technology,  iST-Phospho sample preparation, allows streamlined enrichment of phosphopeptides and an efficient peptide clean-up step. The iST-Phospho method decreases hands-on time and minimises sample loss. Complex workflows previously requiring many different reagents over a XXXXX time period are replaced by a single kit containing all standardised reagents to accomplish phosphoproteomics  preparation for mass spectrometry  protein analysis whether manually, semi or fully automated in a XX  hour time window.

Apply! Simple, streamlined workflows

1.
Lyse, reduce, alkylate
Samples should be liquid, homogenates or a soft pellet. Add LYSE reagent, incubate for 10 mins at 95°C.
2.
Digest
Cool, add DIGEST reagent, incubate for 60 mins at 37°C.
3.
Phospho enrichment
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4.
Purify
Load onto cartridge, WASH and ELUTE. Dry and resuspend in LOAD.
5.
MS analysis
Inject and analyse to see improved peptide identities.
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For technical data and performance statistics see the iST-Phospho Application note

Time flies with PreOmics iST sample preparation

While standard proteomics sample preparation takes around 44 hours – the same time as flying around the world non-stop, PreOmics kits take the same time as flying from our Munich HQ to London or from Boston to Chicago.  

Increase your sample throughput, improve quality and produce reliable data. 

It’s as easy as i-S-T!
PreOmics iST-NHS kit
The products displayed are for visual representation only and may differ from those received

iST-Phospho

iST-Phospho 12x
P.O.000XX
12 sample kit
10 €
iST-Phospho 96x
P.O.000XX
96 sample kit
10 €
Racked into 96 well plate, has collars for individual processing
iST-Phospho HT 192x
P.O.000XX
192 sample kit
10 €
Reagents in amber bottles for easy use with liquid handlers
iST-Phospho PreON 12x
P.O.000XX
12 sample kit
10 €
With PreON plasticware included
iST-Phospho PreON 96x
P.O.000XX
96 sample kit
10 €
With PreON plasticware included

PreOmics iST-Phospho Values

Smile

Improved sample quality

High reproducibility: XXX
Working range: XXX
Increased peptide and protein identifications

Relax

Quicker methodology

XX hands-on time
Single step incorporating lysis, reduction and alkylation
All-in-one solution, all reagents provided
Compatible with standard lab equipment

Realise

Cleaner peptides ready for analysis

Dual clean up step removes hydrophilic and hydrophobic contaminants
Achieve ready-to-measure peptides
Reduce mass spectrometry downtime
Manual to fully automated processing options available

Imagine

Applying the science
Find out more about the wide range of applications and sample
types to which PreOmics technology has been successfully applied