Model organisms such as bacteria, yeast or flies can be entirely processed with our iST or iST-NHS kits. Model organisms are used for a wide range experiments from pathway elucidation, pharmadynamics however model organisms may also be used in a production environment and the efficiency may need monitoring.
The iST/iST-NHS kits are suitable for use in research and regulated environments. The kits contain all you need for your peptide or protein sample preparation and are suitable for use by all members of your lab, no specialist experience is needed. The flexible formats of single samples or racked in to 96 well holders allow the kits to be used manually or with a range of automation options. The cartridges can be processed by centrifugation or using positive pressure manifolds, again giving options for all labs. Dedicated resources are shown below and our technical support team would be happy to help with advice.
For effective disruption and lysis we recommend mechanical force disruption, such as the Diagenode Bioruptor Pico, that allows direct placement of our cartridges, in the presence of our lysis buffers. In addition, many different mechanical force methods can be employed such as traditional bead milling, liquid nitrogen grinding, or commercial systems from various vendors (e.g. Bertin Instruments, Covaris, Hielscher, MP Biomedicals).