Products/iST-Trap

Smile! Immunoprecipitation sample preparation simplified

Fast, reliable, preparation from immunoprecipitated samples ready for mass spectrometry

The challenge:

Integrating immunoprecipitation with multi -step methods of proteomics sample preparation for mass spectrometry analysis has inherent challenges of time, contamination and sample loss. Preparing labelled samples over extended period with a complex method leads to variation in results especially when working with high sample throughput and small starting amounts of input material.

The solution:

The PreOmics / ChromoTek iST-Trap Kit enables the fast and efficient immunoprecipitation of GFP-Fusion proteins and the interaction factors followed by the innovative sample preparation for mass spectrometry based proteomics. All in less than 4 hours.

Apply! Simple, streamlined workflows

1.
Sample immunoprecipitation
Samples should be liquid, add GFP-Trap_A and incubate at 4°C for 60 mins.
2.
Denature, reduce and alkylate
Add LYSE reagent, incubate for 10 mins at 60°C.
3.
Digest
Cool, add DIGEST reagent, incubate for 60 mins at 37°C.
4.
Purify
Load onto cartridge, WASH and ELUTE. Dry and resuspend in LOAD.
5.
MS analysis
Inject and analyse to see improved peptide identities.
For technical data and performance statistics see the iST GFP-Trap Application note here

Time flies with PreOmics iST sample preparation

While standard proteomics sample preparation takes around 44 hours – the same time as flying around the world non-stop, PreOmics kits take the same time as flying from our Munich HQ to London or from Boston to Chicago.  

Increase your sample throughput, improve quality and produce reliable data. 

It’s as easy as i-S-T!
The products displayed are for visual representation only and may differ from those received

iST-Trap

iST-Trap
For iST-Trap kits please visit our partners site

PreOmics iST-Trap Values

Smile

Improved immunoprecipitation sample quality

Reliable identification of GFP-fusion proteins and interactomes
Working range: 1-100 µg
Increased peptide and protein identifications

Relax

Quicker methodology

Less than 4 hours hands-on time
Single step incorporating lysis, reducation and alkylation
All-in-one solution, all reagents provided
Compatible with standard lab equipment

Realise

Cleaner peptides ready for analysis

Dual clean up step removes hydrophilic and hydrophobic contaminants
Achieve ready-to-measure peptides
Reduce mass spectrometry downtime
Range of processing options

Imagine

Applying the science
Find out more about the wide range of applications and sample
types to which PreOmics technology has been successfully applied