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The challenge:
Preparing samples with complex proteomes e.g. human cell lines or tissues, or samples with a wide dynamic range in protein abundances for in depth proteomic characterizations is a compromise between effort, cost and method complexity.
The solution:
The PreOmics® iST-Fractionation Add-on is based on dipole-moment and mixed-phase interactions (patent pending). Samples are prepared using the PreOmics® iST kits until the WASH step and then fractionated using PreOmics® iST-Fractionation Add-on kit. The fractionation buffers are prepared ready for use and the method takes just 10 minutes. The resulting fractions provide a significant increase in the peptide and protein identifications from a single sample.
Apply! Simple, streamlined workflows
1. Samples
Follow iST or iST-NHS protocol until WASH steps.
2. Fraction-1
Swap to a collection container. Add FRACTION-1 to the cartridge and spin.
3. Fraction-2
Change to a new collection container. Add FRACTION-2 to the cartridge and spin.
4. Fraction-3
Change to a new collection container. Add FRACTION-3 to the cartridge and spin.
5. MS analysis
Dry the samples and resuspend them in LC-LOAD. Inject and analyze to see improved peptide and protein identifications.
Results
PreOmics®'
iST-Fractionation Add-on
values
Smile
Improved sample quality
- Achieve proficient peptide fractionation easily
- Working range: 1-100 µg
- Typically 40-50% more protein identifications compared to an unfractionated sample*
* Depending on sample type and MS instrumentation
Relax
Quicker methodology
- 10 min hands-on time
- All-in-one solution, all buffers and consumables provided
- Compatible with standard lab equipment
- Shipped at RT🌿
Realize
Increased peptide and protein identifications
- Achieve ready-to-measure fractionated peptides
- Sensible compromise between processing time and proteomic depth
- Suitable for a range of processing options
iST-Fractionation Add-on
iST-Fractionation Add-on
Request quote
Instrument
Instrument
Simple, fast and reproducible peptide fractionation giving increased proteomic depth
iST-Fractionation Add-on
Request quote
Instrument
Instrument
Simple, fast and reproducible peptide fractionation giving increased proteomic depth
Request quote
8x3 reactions iST-Fractionation Add-on
P.O.00100
8-sample kit
$91
78 €
12x3 reactions iST-Fractionation Add-on
P.O.00101
12-sample kit
$136
118 €
96x3 reactions iST-Fractionation Add-on
P.O.00102
96-sample kit
$639
521 €
The PreOmics® products displayed are for visual representation only and may differ from those received
Resources
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Applying the science
Find out more about the wide range of applications to which PreOmics technology has been successfully applied
