Products/iST-Fractionation Add-on

Smile! Peptide fractionation simplified

Simple, fast and reproducible peptide fractionation giving increased proteomic depth

The challenge:

Preparing samples with complex proteomes e.g. human cell lines or tissues, or samples with a wide dynamic range in protein abundances for in depth proteomic characterizations is a compromise between effort, cost and method complexity.

The solution:

The PreOmics iST-Fractionation Add-on is based on dipole-moment and mixed-phase interactions (patent pending). Samples are prepared using the PreOmics iST kits until the WASH step and then fractionated using PreOmics iST-Fractionation Add-on kit. The fractionation buffers are prepared ready for use and the method takes just 10 minutes. The resulting fractions provide a significant increase in the peptide and protein identifications from a single sample.

Apply! Simple, streamlined workflows

1.
Samples
Follow iST or iST-NHS protocol until WASH steps
2.
Fraction-1
Swap to a collection container. Add FRACTION-1 to cartridge and spin.
3.
Fraction-2
Change to a new collection container. Add FRACTION-2 to cartridge and spin.
4.
Fraction-3
Change to a new collection container. Add FRACTION-3 to cartridge and spin.
5.
MS analysis
Dry the samples and resuspend in LC-LOAD. Inject and analyse to see improved peptide and protein identifications.
For technical data and performance statistics see the iST-Fractionation technical note here

Results

Figure 1: Using 6x105 HeLa cells as starting sample. The left graph shows protein identifications for the three fractions each vs total proteins found for the three fractions combined vs the amount of protein identifications for an unfractionated sample, similarly the right graph shows the same information for the peptide identifications.

Figure 2: Using human plasma (2 µL) as starting sample. The left graph shows protein identifications for the three fractions each vs total proteins found for the three fractions combined vs the amount of protein identifications for an unfractionated sample, similarly the right graph shows the same information for the peptide identifications.

The products displayed are for visual representation only and may differ from those received

iST-Fractionation Add-on

8x3 reactions iST-Fractionation Add-on
P.O.00100
8 sample kit
60 € | 70 $
12x3 reactions iST-Fractionation Add-on
P.O.00101
12 sample kit
90 € | 105 $
96x3 reactions iST-Fractionation Add-on
P.O.00102
96 sample kit
400 € | 490 $

PreOmics iST-Fractionation Add-on Values

Smile

Improved sample quality

Achieve proficient peptide fractionation easily
Working range: 1-100 µg
Typically 40-50% more protein identifications compared to an unfractionated sample*

* Depending on sample type and MS instrumentation

Relax

Quicker methodology

10 min hands-on time
All-in-one solution, all buffers and consumables provided
Compatible with standard lab equipment

Realize

Increased peptide and protein identifications

Achieve ready-to-measure fractionated peptides
Sensible compromise between processing time and proteomic depth
Suitable for a range of processing options

Imagine

Applying the science
Find out more about the wide range of applications and sample
types to which PreOmics technology has been successfully applied