Smile! Peptide fractionation simplified

Fast, reliable, reproducible peptide fractionation ready for proteomic analysis

The challenge:

Maximising number of peptide and protein identifications alongside multi -step methods of proteomics sample preparation for mass spectrometry analysis can be a compromise between speed, ease of use and data.

The solution:

PreOmics iST-Fractionation kit, allows streamlined fractionation of peptides after preparation using any PreOmics iST kit. The iST-fractionation kit provides 3 elution buffers to accomplish peptide fractionation ready for mass spectrometry.  The fractionation method takes approximately 1 hour.

Apply! Simple, streamlined workflows

Samples should be predigested if appropriate and with pH
Load and wash
WASH according to iST kit, spin after each addition, ensure a waste plate is in place.
Swap to a collection plate, add ELUTE 1 to catridge and spin. Repeat with ELUTE 2 and ELUTE 3.
Dry and resuspend
Dry the samples and resuspend in LOAD.
MS analysis
Inject and analyse to see improved peptide identities.
No items found.
For technical data and performance statistics see the iST Fractionation Application note here

Time flies with PreOmics iST sample preparation

While standard proteomics sample preparation takes around 44 hours – the same time as flying around the world non-stop, PreOmics kits take the same time as flying from our Munich HQ to London or from Boston to Chicago.  

Increase your sample throughput, improve quality and produce reliable data. 

It’s as easy as i-S-T!
PreOmics iST-NHS kit
The products displayed are for visual representation only and may differ from those received


iST Fractionation 8x
8 sample kit
10 €
iST-Fractionation 96x
96 sample kit
10 €
Racked into 96 well plate, has collars for individual processing
iST-Fractionation HT 192x
192 sample kit
10 €
Reagents in amber bottles for easy use with liquid handlers
iST Fractionation PreON 12x
12 sample kit
10 €
With PreON plasticware included
iST Fractionation PreON 96x
96 sample kit
10 €
With PreON plasticware included

PreOmics iST-Fractionation Values


Improved sample quality

High reproducibility - do we have this info?
Working range: 1-100 µg - is this right?
Need quantity e.g. 3 times more  peptide  identifications


Quicker methodology

XX hands-on time
All-in-one kits, all reagents provided
Compatible with standard lab equipment


Cleaner peptides ready for analysis

Dual clean up step removes hydrophilic and hydrophobic contaminants
Achieve ready-to-measure peptides
Reduce mass spectrometry downtime
Suitable for a range of processing options


Applying the science
Find out more about the wide range of applications and sample
types to which PreOmics technology has been successfully applied