Tissue homogenates and cell lysates are the starting point for a wide variety of downstream applications in genomics and proteomics. The extracted biomolecules are further characterized with methods, such as genotyping, immunoassays, and mass spectrometry, in order to identify biomarkers or understand cellular pathways.
Particularly proteomics requires thoroughly homogenized samples to obtain high protein yields even from small inputs like needle biopsies for sensitive and quantitative analyses. Furthermore, standardization, reproducibility, and parallelization are key to the processing of large numbers of samples in high-throughput environments. However, scientists will require a homogenization method that allows for high flexibility and versatility to cover a broad range of projects.
BeatBox is a dedicated system for convenient, semi-automated sample homogenization of many tissue types and cells. Flexible regarding input amounts from 1-50 mg, it consistently delivers reproducible results even for challenging downstream analyses like LC-MS-based proteomics with scalable throughput from 1-96 samples.
1-2 mg of four different mouse tissues were homogenized using the BeatBox and two other methods. Results clearly show the same or increased protein yield using BeatBox comparatively with good reproducibility evidenced by the %CV results.
"Having such a quiet, compact tissue preparation solution on our bench day by day can only further serve to increase our efficiency."
Excellent reproducibility, even for challenging samples
1-96 samples, compatible with both 96 well plates and single tubes
Compact and quiet instrument for beginners and experts alike
Xylene-free approach without separate deparaffinization step