Products/Agilent

Smile! Protein and peptide sample preparation automated

Applications for Agilent Bravo

The challenge:

How do you bring further standardization, reproducibility and hands on time savings to proteomics sample preparation for  LC-MS analysis when you have already successfully addressed  the many challenges of time, sample contamination, sample loss and reproducibility?

The solution:

Automate! In conjunction with Agilent, PreOmics' kits are suitable for use on the Agilent Bravo and Agilent AssayMAP Bravo platforms. Meeting the demands of reproducible, robust and sensitive proteomics sample preparation for LC-MS analysis, these applications allow research labs to partially or fully automate proteomics processing, decreasing the hands on time.

Relax! iST compatible workflows

1.
Adding samples
Samples should be liquid, homogenates or a soft pellet.
2.
iST kit set up
Add the appropriate iST kit components to Bravo AssayMap.
3.
AssayMAP automated sample preparation
Select the appropriate protocol and start.
4.
Mass spectrometric analysis
Inject samples into your LC-MS system.
5.
Data analysis
Process your data to see improved peptide identities.
Using automation significantly reduces technical variability of proteomics sample preparation by reducing manual steps and eliminating processing errors. Spend less time preparing your samples while achieving better results.

Results

Should be below the results figures. There are two options when using Agilent automation, the partially automated Agilent Bravo which requires off-board centrifugation and Agilent AssayMAP Bravo which is a fully automated (application note coming soon). The figures above show data generated on the Agilent Bravo in a partially-automated protocol, using 1 µL plasma with analysis on a Thermo Q-Exactive HF Orbitrap

PreOmics Values

Smile

Improved sample quality

High reproducibility R=0.98
Working range: 1-100 µg
Increased peptide and protein identifications

Relax

Sample preparation automated

Automation option for label-free workflow
Reduces manual processing steps
Generates standardized and highly reproducible proteomics LC-MS results
Applications for the PreOmics protocols

Realise

Cleaner peptides ready for analysis

Dual clean up step removes hydrophilic and hydrophobic contaminants
Achieve ready-to-measure peptides
Reduce mass spectrometry downtime

Imagine

Applying the science
Find out more about the wide range of applications and sample
types to which PreOmics technology has been successfully applied