Products/Agilent

Smile! Protein and peptide sample preparation automated

Applications for Agilent Bravo

The challenge:

How do you bring further standardization, reproducibility and hands on time savings to proteomics sample preparation for  LC-MS analysis when you have already successfully addressed  the many challenges of time, sample contamination, sample loss and reproducibility?

The solution:

In conjunction with Agilent, PreOmics' kits are suitable for use on the Agilent Bravo platform. Meeting the demands of reproducible, robust and sensitive proteomics sample preparation for LC-MS analysis, these applications allow research labs to partially automate proteomics processing, decreasing the hands on time.

Relax! iST compatible workflows

1.
Adding samples
Samples should be liquid, homogenates or a soft pellet.
2.
iST kit set up
Add the appropriate iST kit components to Agilent Bravo.
3.
Sample preparation
Protocols need to be programmed by an in house specialist.
4.
Mass spectrometric analysis
Inject samples into your LC-MS system.
5.
Data analysis
Process your data to see improved peptide identities.
Using automation significantly reduces technical variability of proteomics sample preparation by reducing manual steps and eliminating processing errors. Spend less time preparing your samples while achieving better results.

Results

The partially automated Agilent Bravo requires off-board centrifugation. The figures above show data generated on the Agilent Bravo in a partially-automated protocol, using 1 µL plasma with analysis on a Thermo Q-Exactive HF Orbitrap

PreOmics Values

Smile

Improved sample quality

High reproducibility R2=0.9
Working range: 1-100 µg
Increased peptide and protein identifications

Relax

Sample preparation automated

Reduces manual processing steps
Generates standardized and highly reproducible proteomics LC-MS results
Applications for the PreOmics protocols

Realise

Cleaner peptides ready for analysis

Dual clean up step removes hydrophilic and hydrophobic contaminants
Achieve ready-to-measure peptides
Reduce mass spectrometry downtime

Imagine

Applying the science
Find out more about the wide range of applications and sample
types to which PreOmics technology has been successfully applied